In recent years, the concepts of Industry 4.0 and even Industry 5.0 as well as the rapidly developing technology have brought about big opportunities and challenges for businesses. The aim of the study is to find out the Industry 4.0 conceptual awareness levels of students who are prospective managers and reveal the effects of the management information systems course, which is included in the department curriculum, on this awareness. It is also aimed in the study to find out whether the students’ levels of industry 4.0 conceptual awareness vary across variables such as gender, working status outside school, the sector where they plan to work after school, grade point average (GPA) and type of high school the participants graduated from. The research population is comprised of students studying in the department of Business Administration in the Faculty of Political Sciences in a public university. The 25 students at 4th grade in the research sample who have taken the Management Information Systems course were chosen via convenience sampling method which is among non-probability sampling methods. Uncontrolled pre-test-post-test model was used in the study. Validity and reliability analyses of the scales as well as normality analysis, Mann-Whitney U test, Kruskal-Wallis H test and dependent samples t test were performed in the study. As a result of the analyses, it was found out that students’ industry 4.0 conceptual awareness levels do not differ depending on gender, working status outside school, the sector where they plan to work after school, grade point average (GPA) type of high school the participants graduated from. On the other hand, average scores for pre-test and post-test regarding the students’ industry 4.0 conceptual awareness levels revealed a significant increase between average pre-test scores (x= 2,97) and average post-test scores (x= 3,36). When the pre-test and post-test scores were compared, “Artificial Intelligence”, “Virtual Reality”, “Wearable Technologies”, “Unmanned Systems”, “Customizable Product Development”, “Learning (Smart) Robots”, “Cyber Security” and “3D Printers” were among the top fields about which the students had the highest level of awareness